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AN177: Separation of an Intact Monoclonal Antibody and Fractionation of Monoclonal Antibody Papain Digest Fragments Using IMAC: Papain Digest Separation
Description
This application note describes a method for fractionating IgG1 MAb Fc and Fab fragments with the ProPac IMAC-10 column preloaded with copper and analyzing the fractions using the ProPac WCX-10 column. This record reports the method for the papain digest separation with the ProPac IMAC-10 column.| Market: | BioPharma |
| Keywords: | FAB, Fc, IgG, Immobilized Metal Affinity Chromatography, mAb, MAB, ProPac, ProPac IMAC-10, ProPac WCX-10 |
| Matrix: | Protein |
| Author: | Brian De Borba and Jeff Rohrer |
| Affiliation: | Thermo Fisher Scientific |
For Research Use Only. Not for use in diagnostic procedures.
