Selecting buffers to remove uncertainty in tryptic digestion
DescriptionThe Thermo Scientific SMART Digest kit was used for the digestion of insulin. A variety of common buffer additives were compared to the SMART Digest buffer. Samples were analyzed on a Thermo Scientific Dionex UltiMate 3000 RSLC system via reversed-phase separation on a Thermo Scientific Accucore C18 column, with UV detection at 280 nm.
|Keywords:||Accucore C18, MAb, Peptides, Proteins, Trypsin digest, Insulin, SMART Digest, Buffers|
|Affiliation:||Thermo Fisher Scientific|
For Research Use Only. Not for use in diagnostic procedures.