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Selecting buffers to remove uncertainty in tryptic digestion Be the first to rate this application


The Thermo Scientific SMART Digest kit was used for the digestion of insulin. A variety of common buffer additives were compared to the SMART Digest buffer. Samples were analyzed on a Thermo Scientific Dionex UltiMate 3000 RSLC system via reversed-phase separation on a Thermo Scientific Accucore C18 column, with UV detection at 280 nm.
Market: BioPharma
Keywords: Accucore C18, MAb, Peptides, Proteins, Trypsin digest, Insulin, SMART Digest, Buffers
Matrix: N/A
Author: Valeria Barattini
Affiliation: Thermo Fisher Scientific
Uploaded on 6/29/2015.

For Research Use Only. Not for use in diagnostic procedures.