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Selecting buffers to remove uncertainty in tryptic digestion
Description
The Thermo Scientific SMART Digest kit was used for the digestion of insulin. A variety of common buffer additives were compared to the SMART Digest buffer. Samples were analyzed on a Thermo Scientific Dionex UltiMate 3000 RSLC system via reversed-phase separation on a Thermo Scientific Accucore C18 column, with UV detection at 280 nm.| Market: | BioPharma |
| Keywords: | Accucore C18, MAb, Peptides, Proteins, Trypsin digest, Insulin, SMART Digest, Buffers |
| Matrix: | N/A |
| Author: | Valeria Barattini |
| Affiliation: | Thermo Fisher Scientific |
For Research Use Only. Not for use in diagnostic procedures.
