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Selecting buffers to remove uncertainty in tryptic digestion
Instrument Type: HPLCThe Thermo Scientific SMART Digest kit was used for the digestion of insulin. A variety of common buffer additives were compared to the SMART Digest buffer. Samples were analyzed on a Thermo Scientific Dionex UltiMate 3000 RSLC system via reversed-phase separation on a Thermo Scientific Accucore C18 column, with UV detection at 280 nm.