Concentration determination of a Bevacizumab harvest sample by using an Affinity capture Protein-A column

Instrument Type: HPLC (Biocompatible)

The Thermo Scientific Dionex UltiMate 3000 LC Bio-LC system is applied for the affinity analysis for concentration determination of Bevacizumab Harvest Sample. The analysis was performed on a Thermo Scientific MabPac Protein A column with a UV detection at 280 nm. With a run time of 3.0 minutes, the method allows easy and fast estimation of Protein content in harvest sample of Bevacizumab. Linearity from 0.05-2.0 mg/mL with RSD of 0.5% (n=3)

Four high resolution peptide maps using a Thermo Scientific Acclaim PolarAdvantage II HPLC column

Instrument Type: HPLC

The Thermo Scientific UltiMate 3000 RSLC system is applied for the analysis of ovalbumin, fetuin, β-casein and bovine serum albumin. The separation is performed on a Thermo Scientific Acclaim PolarAdvantage II HPLC column, with UV detection at 210 nm.