Direct measurement of sialic acids from glycoprotein hydrolysis by HPLC-CAD

Instrument Type: HPLC

A isocratic method was developed for determine sialic acids content in glycoprotein using Thermo Scientific U3000 HPLC system with charged aerosol detector. Sialic acids were released from human and bovine transferrin with enzyme digestion and seperated on a Dionex Trinity P2 column without derivatization. N-acetylneuraminic acid and N-glycolyneuraminic acid can be determined in less than 12 min with this method. Sample solvent: 40% 100mM ammonium acteate at pH5, 60% acetonitrile

AN253: HPAE-PAD Determination of Infant Formula Sialic Acid

Instrument Type: IC

Sialic acids in infant formulas are accurately determined by HPAE-PAD using the Dionex CarboPac PA20 column following acid hydrolysis and maltodextrin removal using one of two sample-preparation methods. HPAE-PAD provides reliable determination of sialic acids in acid hydrolyzed infant formula samples without sample derivatization. This method may be used to quantify sialic acids in formulas that have been enriched with sialic acids.

AN215: Separation of Asparagine-Linked (N-Linked) Oligosaccharides from Human Polyclonal IgG

Instrument Type: IC

This application note shows how the PA200 improves N-linked oligosaccharide separations. The PA200 is used to profile N-linked oligosaccharides released from human polyclonal IgG by the enzyme PNGase F or endoglycosidase H (Endo H). This note also shows how subsequent exoglycosidase digestions can be used to assist in understanding and identifying oligosaccharide structure.