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Displaying 1-3 of 3 results for Tag: Endoglycosidase
TN36: Analysis of Exoglycosidase Digestions of N-Linked Oligosaccharides Using HPAE-PAD (Part C)
Instrument Type: ICThis technical note describes the use of exoglycosidases to obtain structural information about N-linked oligosaccharides commonly found in mammalian glycoproteins. HPAE-PAD is used to confirm the identity of the released monosaccharides, as well as the remaining di-, tri,- or oligosaccharides. Empirical rules used to predict oligosaccharide elution order are included in the last section of this technical note.
TN36: Analysis of Exoglycosidase Digestions of N-Linked Oligosaccharides Using HPAE-PAD (Part B)
Instrument Type: ICThis technical note describes the use of exoglycosidases to obtain structural information about N-linked oligosaccharides commonly found in mammalian glycoproteins. HPAE-PAD is used to confirm the identity of the released monosaccharides, as well as the remaining di-, tri,- or oligosaccharides. Empirical rules used to predict oligosaccharide elution order are included in the last section of this technical note.
AN215: Separation of Asparagine-Linked (N-Linked) Oligosaccharides from Human Polyclonal IgG
Instrument Type: ICThis application note shows how the PA200 improves N-linked oligosaccharide separations. The PA200 is used to profile N-linked oligosaccharides released from human polyclonal IgG by the enzyme PNGase F or endoglycosidase H (Endo H). This note also shows how subsequent exoglycosidase digestions can be used to assist in understanding and identifying oligosaccharide structure.
