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Displaying 1-5 of 5 results for Tag: Tryptic digest
SMART Digest compared to classic in-solution digestion of rituximab for in-depth peptide mapping characterizationInstrument Type: LCMS
This study compares the newly developed Thermo Scientific™ SMART Digest™ kit to classic in-solution protein digestion methods, focusing on protein sequence coverage and identified post-translational modifications (PTMs), including deamidation, oxidation, and glycosylation. A Thermo Scientific™ Acclaim™ VANQUISH™ C18 column with conventional water/acetonitrile-based gradients and the Thermo Scientific™ Vanquish™ Flex UHPLC system were used for separation in combination with the Thermo Scientific™ Q Exactive™ HF Hybrid Quadrupole-Orbitrap™ mass spectrometer.
Reliable Results in Peptide Mapping Using the Vanquish Flex UHPLC SystemInstrument Type: UHPLC
Peptide mapping is one of the major analyses in biotherapeutics characterization. Here we show peptide mapping with the Thermo Scientific Vanquish Flex UHPLC system. An efficient and reliable separation of peptides obtained from a bovine serum albumin (BSA) digest is done on an Thermos Scientific Acclaim RSLC 120, C18, 2.2 µm Analytical column. Excellent retention time and area precisions allow reliable qualitative and quantitative data interpretation.
Comparison of two reversed-phase columns for the analysis of tryptic digests of proteinsInstrument Type: HPLC
The Thermo Scientific UltiMate 3000 RSLC system is applied for the analysis of tryptic digests of proteins. The separation is performed on Thermo Scientific Acclaim RSLC C18 and Acclaim RSLC PolarAdvantage II 2.2µm columns, with UV detection at 210 nm.
Increased resolution of tryptic digests of proteins using a Thermo Scientific Acclaim RSLC C18 HPLC columnInstrument Type: UHPLC
In this work, a digestion of bovine serum albumin is resolved using the Thermo Scientific Acclaim RSLC C18 into 127 peaks with an average peak width of 0.27 minutes. For a 90 minute gradient, this works out to a theoretical peak capacity of >300.
Improved analysis of a b-lactoglobulin tryptic digest using a Thermo Scientific Hypersil GOLD HPLC columnInstrument Type: HPLC
The Thermo Scientific HPLC system is applied for the analysis of a b-Lactoglobulin tryptic digest. The separation is performed on a Thermo Scientific Hypersil GOLD HPLC column, with UV detection at 220 nm.