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Reliable Results in Peptide Mapping Using the Vanquish Flex UHPLC System Be the first to rate this application

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271217222732Time [min]-20020406080100120Response [mAU]
# Compound Name Compound Class RT (min)
1 Component 1 Peptide 2.50
2 Component 17 Peptide 2.64
3 Component 31 Peptide 2.81
4 Component 34 Peptide 2.95
5 Component 35 Peptide 3.18
6 Component 38 Peptide 4.62
7 Component 43 Peptide 4.77
8 Component 44 Peptide 4.94
9 Component 45 Peptide 5.22
10 Component 47 Peptide 5.95
11 Component 48 Peptide 6.04
12 Component 53 Peptide 7.05
13 Component 54 Peptide 7.46
14 Component 61 Peptide 7.61
15 Component 63 Peptide 7.81
16 Component 64 Peptide 7.98
17 Component 73 Peptide 8.34
18 Component 75 Peptide 8.46
19 Component 76 Peptide 8.55
20 Component 82 Peptide 8.70
21 Component 83 Peptide 8.84
22 Component 88 Peptide 9.28
23 Component 90 Peptide 9.69
24 Component 93 Peptide 9.85
25 Component 96 Peptide 10.01
26 Component 97 Peptide 10.24
27 Component 98 Peptide 10.37
28 Component 100 Peptide 10.50
29 Component 104 Peptide 10.70
30 Component 109 Peptide 10.86
31 Component 115 Peptide 11.17
32 Component 117 Peptide 11.29
33 Component 120 Peptide 11.48
34 Component 133 Peptide 12.25
35 Component 135 Peptide 12.71
36 Component 136 Peptide 13.01
37 Component 137 Peptide 13.19
38 Component 142 Peptide 13.29
39 Component 146 Peptide 13.55
40 Component 160 Peptide 13.91
41 Component 161 Peptide 13.96
42 Component 162 Peptide 14.22
43 Component 164 Peptide 14.41
44 Component 174 Peptide 14.53
45 Component 177 Peptide 15.10
46 Component 183 Peptide 15.40
47 Component 186 Peptide 15.54
48 Component 192 Peptide 15.63
49 Component 193 Peptide 15.66
50 Component 194 Peptide 15.71
51 Component 195 Peptide 15.84
52 Component 196 Peptide 16.03
53 Component 197 Peptide 16.12
54 Component 198 Peptide 16.34
55 Component 199 Peptide 16.48
56 Component 200 Peptide 16.65
57 Component 201 Peptide 16.79
58 Component 202 Peptide 16.92
59 Component 203 Peptide 17.07
60 Component 204 Peptide 17.18
61 Component 205 Peptide 17.24
62 Component 206 Peptide 17.32
63 Component 207 Peptide 17.47
64 Component 208 Peptide 17.67
65 Component 114 Peptide 18.04
66 Reference Peak Peptide 18.34
67 Component 213 Peptide 18.52
68 Component 214 Peptide 18.62
69 Component 215 Peptide 18.89
70 Component 216 Peptide 19.00
71 Component 217 Peptide 19.11
72 Component 218 Peptide 19.17
73 Component 219 Peptide 19.25
74 Component 220 Peptide 19.51
75 Component 221 Peptide 20.00
76 Component 222 Peptide 20.11
77 Component 223 Peptide 20.24
78 Component 224 Peptide 20.44
79 Component 226 Peptide 20.70
80 Component 227 Peptide 21.00
81 Component 229 Peptide 21.22
82 Component 230 Peptide 21.63
83 Component 231 Peptide 21.81
84 Component 232 Peptide 21.88
85 Component 233 Peptide 22.05
86 Component 235 Peptide 22.28
87 Component 236 Peptide 22.47
88 Component 238 Peptide 22.91
89 Component 239 Peptide 23.03
90 Component 240 Peptide 23.25
91 Component 241 Peptide 23.38
92 Component 242 Peptide 23.50
93 Component 243 Peptide 23.64
94 Component 244 Peptide 23.83
95 Component 245 Peptide 24.13
96 Component 247 Peptide 24.38
97 Component 249 Peptide 24.71
98 Component 250 Peptide 24.78
99 Component 251 Peptide 24.88
100 Component 252 Peptide 25.07
101 Component 253 Peptide 25.19
102 Component 254 Peptide 25.51
103 Component 255 Peptide 25.93
104 Component 256 Peptide 26.14
105 Component 257 Peptide 26.26
106 Component 258 Peptide 26.49
107 Component 259 Peptide 26.61
108 Component 260 Peptide 26.95
109 Component 262 Peptide 27.28
110 Component 263 Peptide 27.34
111 Component 267 Peptide 28.50
112 Component 268 Peptide 29.24
113 Component 269 Peptide 29.32
114 Component 270 Peptide 29.44
115 Component 271 Peptide 29.98
116 Component 272 Peptide 30.35

Description

Peptide mapping is one of the major analyses in biotherapeutics characterization. Here we show peptide mapping with the Thermo Scientific Vanquish Flex UHPLC system. An efficient and reliable separation of peptides obtained from a bovine serum albumin (BSA) digest is done on an Thermos Scientific Acclaim RSLC 120, C18, 2.2 µm Analytical column. Excellent retention time and area precisions allow reliable qualitative and quantitative data interpretation.
Market: BioPharma
Keywords: Biocompatible, Biotherapeutics, Monoclonal antibody, Protein digest, Acclaim C18 RSLC column
Matrix: Tryptic digest of BSA
Author: Carsten Paul
Affiliation: Thermo Fisher Scientific
Uploaded on 6/18/2015.

For Research Use Only. Not for use in diagnostic procedures.

Tags

Acclaim C18 RSLC Biocompatible BioPharma Biotherapeutic BSA Monoclonal antibody Peptide Protein digest Tryptic digest UHPLC
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