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Reliable Results in Peptide Mapping Using the Vanquish Flex UHPLC System

Instrument Type: UHPLC

Peptide mapping is one of the major analyses in biotherapeutics characterization. Here we show peptide mapping with the Thermo Scientific Vanquish Flex UHPLC system. An efficient and reliable separation of peptides obtained from a bovine serum albumin (BSA) digest is done on an Thermos Scientific Acclaim RSLC 120, C18, 2.2 µm Analytical column. Excellent retention time and area precisions allow reliable qualitative and quantitative data interpretation.

Improved monitoring of monoclonal antibody heterogeneity by cation-exchange chromatography using a Thermo Scientific ProPac WCX-10 column

Instrument Type: HPLC (Biocompatible)

The Thermo Scientific UltiMate 3000 titanium system is applied for analyzing a humanized IgG1 MAb for C-terminal lysine variants. The separation was performed on a Thermo Scientific ProPac WCX-10 column, with UV detection at 280 nm.