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Determination of Meat Authenticity Using a Comprehensive Targeted Proteomic Strategy and High-Resolution Mass Spectrometry Be the first to rate this application

determination meat authenticity using a comprehensive targeted proteomic strategy highresolution mass spectrometry


We present a summary of a sensitive and robust HRAM LC-MS method using a Thermo Scientific Q Exactive mass spectrometer for the identification and detection of marker proteins in raw meat samples. Four proteotypic peptides were used to perform accurate meat speciation and authenticity. Myoglobin tryptic peptides from each species were detected with an observed m/z below 1.3 ppm. This targeted method allowed for the detection of undesired meat species down to 1% (w/w) of the entire sample.
Market: Food and Beverage
Keywords: Authenticity, HPLC, Meat, Proteomics, UltiMate 3000 RSLC, Food, Q Exactive, Biomarkers, High-resolution mass spectrometry
Matrix: Meat
Author: Complete method: Alberto Ruiz Orduna, et al. (2015) Food Additives & Contaminants: Part A, DOI: 10.1080/19440049.2015.1064173
Affiliation: Département de biomédecine vétérinaire, Faculté de médecine vétérinaire, Université de Montréal; Thermo Fisher Scientific
Uploaded on 12/14/2016.