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Determination of Meat Authenticity Using a Comprehensive Targeted Proteomic Strategy and High-Resolution Mass Spectrometry
| # | Compound Name | Compound Class | Exact Mass (m/z) | Mass Accuracy (ppm) |
|---|---|---|---|---|
| 1 | HPGDFGADAQGAMSK | Peptide | 744.8314 | 1.340 |
| 2 | HPGDFGADAQGAMTK | Peptide | 751.8383 | 0.670 |
| 3 | HPSDFGADAQAAMSK | Peptide | 766.8435 | 0.130 |
| 4 | HPSDFGADAQGAMSK | Peptide | 759.8357 | 0.790 |
Description
We present a summary of a sensitive and robust HRAM LC-MS method using a Thermo Scientific Q Exactive mass spectrometer for the identification and detection of marker proteins in raw meat samples. Four proteotypic peptides were used to perform accurate meat speciation and authenticity. Myoglobin tryptic peptides from each species were detected with an observed m/z below 1.3 ppm. This targeted method allowed for the detection of undesired meat species down to 1% (w/w) of the entire sample.| Market: | Food and Beverage |
| Keywords: | Authenticity, HPLC, Meat, Proteomics, UltiMate 3000 RSLC, Food, Q Exactive, Biomarkers, High-resolution mass spectrometry |
| Matrix: | Meat |
| Author: | Complete method: Alberto Ruiz Orduna, et al. (2015) Food Additives & Contaminants: Part A, DOI: 10.1080/19440049.2015.1064173 |
| Affiliation: | Département de biomédecine vétérinaire, Faculté de médecine vétérinaire, Université de Montréal; Thermo Fisher Scientific |
