High resolution charge variants analysis of recombinant human IgG1K NIST mAb

Instrument Type: HPLC-CAD

The Thermo Scientific Vanquish Flex UHPLC system with UV detection is applied for the charge variants analysis of NISTmAb Humanized IgG1K monoclonal antibody using strong cation exchange-based chromatographic separation on a MAbPac SCX-10 column. The use of Thermo Scientific CX-1 pH gradient buffers enabled high resolution separation of reference material monoclonal antibody charge variants using a 30 minute gradient.

TN25: Determination of Transition Metals in Complex Matrices by Chelation Ion Chromatography

Instrument Type: IC

This technical note describes chelation concentration, a selective concentration method, coupled directly to an ion chromatograph for the determination of transition metals in complex matrices. This method, Chelation Ion Chromatography, combines analyte concentration and matrix elimination with analytical separations and sensitive detection for transition and lanthanide metals. The result is a chromatographic technique that permits trace and ultratrace determination of metals in variety of sample matrices, especially those high in alkali and alkaline earth metals.

AN31: Determination of Anions in Acid Rain by Ion Chromatography

Instrument Type: IC

This application note describes the established ion chromatographic method for the determination of anions in rainwater samples extended to the use of microbore columns and accessories. A rainwater standard from the National Institute of Standards and Technology (NIST) was analyzed, verifying the accuracy of the method. This method is updated in AU146 to include the use of electrolytic eluent generation and the IonPac AS18 column.