Thermo Scientific AppsLab Library: Monitoring of multiple quality attributes of intact monoclonal antibodies from bioreactors by switchable 2D-LC-MS

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Description

The presented 2D-LC-MS system facilitates the straightforward analysis of mAb producing cell culture samples for the upstream monitoring of titer, charge variants, and size variants of the intact antibody. While the first dimension purifies the mAb from matrix components by Protein A affinity chromatography, selectable second dimension mechanisms enable the assessment of the aggregation level by SEC or the abundance monitoring of glycoforms and other variants by SCX.

Market:	BioPharma
Keywords:	Intact protein, Size exclusion, SEC, Cation Exchange, Protein A, SCX, Process control, PAT, charge variants, heart-cut 2D-LC, size variants, antibody, biologics, iMAM
Matrix:	Cell culture
Author:	Maria Grübner, Kai Scheffler, Alexander Schwahn, Aleš Holfeld, Sara Carillo, Jonathan Bones, Ken Cook
Affiliation:	Thermo Fisher Scientific, Germering, Germany / Reinach, Switzerland / Hemel Hempstead, UK; National Institute for Bioprocessing Research and Training (NIBRT), Dublin, Ireland

Uploaded on 10/1/2024.

For Research Use Only. Not for use in diagnostic procedures.