Intact mass analysis of monoclonal antibody charge variants by multi heart-cut 2D-LC/MS coupling ion-exchange and reversed-phase chromatography
DescriptionMultiple heart-cut 2D-LC-MS is an efficient approach for charge variant analysis of antibodies by enabling direct MS analysis of first dimension ion-exchange fractions after desalting in the second dimension. Intact mass analysis facilitates identification and monitoring of mAb charge variant patterns. Annotations of major and minor charge variant peaks could be proposed. Enrichment of one fraction was used to improve the MS signal intensity.
|Glycans, ion exchange, Monoclonal antibody, Protein, Reversed-phase, mass spectrometry, Biopharma, Desalting, Charge Variants, Infliximab, Orbitrap Q Exactive 240, strong cation exchange, intact, top-down, PTMs
|Maria Grübner; Alexander Schwahn; Mauro De Pra
|Thermo Fisher Scientific, Germering, Germany; Thermo Fisher Scientific, Reinach, Switzerland