Separation of Acidic and Basic charge variants of Denosumab by Ion Exchange chromatography using Salt Gradient Method
DescriptionThe Thermo Scientific Dionex UltiMate 3000 LC Bio-LC system is applied for the Ion Exchange analysis of Denosumab. The separation was performed on a Thermo Scientific MabPac SCX-10 column with a UV detection at 220 nm. With a run time of 65 minutes the method allows easy separation of charge variants of Denosumab.
|Keywords:||HPLC, MAbPac SCX-10 Column, Denosumab, PDA detection, Charge Variants, Salt Gradient|
|Affiliation:||Thermo Fisher Scientific, APAC CoE Lab, Ahmedabad, India|
For Research Use Only. Not for use in diagnostic procedures.