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Separation of Acidic and Basic charge variants of Denosumab by Ion Exchange chromatography using Salt Gradient Method
Description
The Thermo Scientific Dionex UltiMate 3000 LC Bio-LC system is applied for the Ion Exchange analysis of Denosumab. The separation was performed on a Thermo Scientific MabPac SCX-10 column with a UV detection at 220 nm. With a run time of 65 minutes the method allows easy separation of charge variants of Denosumab.| Market: | BioPharma |
| Keywords: | HPLC, MAbPac SCX-10 Column, Denosumab, PDA detection, Charge Variants, Salt Gradient |
| Matrix: | N/A |
| Author: | Manish Singh |
| Affiliation: | Thermo Fisher Scientific, APAC CoE Lab, Ahmedabad, India |
For Research Use Only. Not for use in diagnostic procedures.
