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Separation of Acidic and Basic charge variants of Denosumab by Ion Exchange chromatography using pH Gradient Buffers

Instrument Type: HPLC (Biocompatible)

The Thermo Scientific Dionex UltiMate 3000 LC Bio-LC system is applied for the Ion Exchange analysis of Denosumab. The separation was performed on a Thermo Scientific MAbPac SCX-10 and pH gradient buffers with a UV detection at 280 nm. With a run time of 50 minutes the method allows easy separation of charge variants of Denosumab.

Separation of Acidic and Basic charge variants of Denosumab by Ion Exchange chromatography using Salt Gradient Method

Instrument Type: HPLC (Biocompatible)

The Thermo Scientific Dionex UltiMate 3000 LC Bio-LC system is applied for the Ion Exchange analysis of Denosumab. The separation was performed on a Thermo Scientific MabPac SCX-10 column with a UV detection at 220 nm. With a run time of 65 minutes the method allows easy separation of charge variants of Denosumab.