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Separation of Acidic and Basic charge variants of Denosumab by Ion Exchange chromatography using pH Gradient Buffers
Description
The Thermo Scientific Dionex UltiMate 3000 LC Bio-LC system is applied for the Ion Exchange analysis of Denosumab. The separation was performed on a Thermo Scientific MAbPac SCX-10 and pH gradient buffers with a UV detection at 280 nm. With a run time of 50 minutes the method allows easy separation of charge variants of Denosumab.| Market: | BioPharma |
| Keywords: | HPLC, Denosumab, PDA detection, pH gradient, MabPac SCX-10, CX-1 pH Gradient Buffer A, CX-1 pH Gradient Buffer B |
| Matrix: | N/A |
| Author: | Manish Singh |
| Affiliation: | Thermo Fisher Scientific, APAC CoE Lab, Ahmedabad, India |
For Research Use Only. Not for use in diagnostic procedures.
