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Separation of Acidic and Basic charge variants of Denosumab by Ion Exchange chromatography using Salt Gradient Method

Instrument Type: HPLC (Biocompatible)

The Thermo Scientific Dionex UltiMate 3000 LC Bio-LC system is applied for the Ion Exchange analysis of Denosumab. The separation was performed on a Thermo Scientific MabPac SCX-10 column with a UV detection at 220 nm. With a run time of 65 minutes the method allows easy separation of charge variants of Denosumab.