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CAN108: Ion Chromatography Coupled with Mass Spectrometry for Metabolomics
Description
Here, the author describes an ion-exchange system that provides good separation of the polar metabolities with on-line desalting to allow MS detection. This technique will allow studies of key metabolites that do not separate well on traditional reversed-phase columns. Some ionic metabolites may be positional isomers adjacent to each other in a synthetic pathway and as such, are isobaric. In such cases, MS is not sufficient to differentiate the two compounds. There is clearly a need for adequate separation techniques for isomeric metabolites.| Market: | Metabolomics |
| Keywords: | ADP, AMP, ATP, Pyruvate, dGTP, dCTP, dTTP, Citrate, Succinate, Isocitrate, IonPac AS11, 6-phosphogluconate, CAN108, FDP, Glyoxylate, Phosphoenol pyruvate, Robose 5-phosphate, Sedo-heptulose 7-phosphate, Tryptophan pathway, Amino acid metabolism |
| Matrix: | Water |
| Author: | Karl Burgess |
| Affiliation: | Functional Genomics, Joesef Black building, Glasgow University, Glasgow, UK |
For Research Use Only. Not for use in diagnostic procedures.
