CAN109: Primary Metabolite Analysis of Plant Material Using a Triple Quadrupole MS Coupled to a Monolith Anion-Exchange Column

Instrument Type: ICMS

A platform using Dionex ion chromatography (IC) coupled to triple quadrupole mass spectrometry (MS) was developed to measure various metabolites in Brassica napus seeds. The IC tandem mass spectrometry (IC-MS/MS) technique provides a good tool to identify and quantify metabolites in a complex matrix.

CAN108: Ion Chromatography Coupled with Mass Spectrometry for Metabolomics

Instrument Type: ICMS

Here, the author describes an ion-exchange system that provides good separation of the polar metabolities with on-line desalting to allow MS detection. This technique will allow studies of key metabolites that do not separate well on traditional reversed-phase columns. Some ionic metabolites may be positional isomers adjacent to each other in a synthetic pathway and as such, are isobaric. In such cases, MS is not sufficient to differentiate the two compounds. There is clearly a need for adequate separation techniques for isomeric metabolites.

Fast analysis of nucleotides using a Thermo Scientific Accucore aQ HPLC column

Instrument Type: HPLC

The Thermo Scientific HPLC system is applied for the analysis of nucleotides. The separation is performed on a Thermo Scientific Accucore aQ HPLC column, with UV detection at 260 nm.