Determinations of organic acids in fruit juices are used by the beverage industry for flavor characterization, identification of spoilage, identification of adulteration by a less costly juice, and product labeling. In this study, inorganic anions and organic acids in diluted filtered apple and orange juice samples were determined on a Dionex IonSwift MAX-100 anion-exchange column using electrolytically generated hydroxide gradient from 0.1–65 mM KOH over 25 min at 15 μL/min.

Here, the author describes an ion-exchange system that provides good separation of the polar metabolities with on-line desalting to allow MS detection. This technique will allow studies of key metabolites that do not separate well on traditional reversed-phase columns. Some ionic metabolites may be positional isomers adjacent to each other in a synthetic pathway and as such, are isobaric. In such cases, MS is not sufficient to differentiate the two compounds. There is clearly a need for adequate separation techniques for isomeric metabolites.

Fermentation broths are complex mixtures of nutrients, waste products, cells and cell debris, and desired products. Fermentation monitoring is important for many industries because carbon sources and metabolic by-products can impact the yield of the desired products. This application note describes the use of two different anion-exchange columns, IonPac AS11 and IonPac AS11-HC, with suppressed conductivity detection, to analyze common organic and inorganic anions in yeast and bacterial fermentation broths.

One of the most important applications of IC in clinical chemistry is the analysis of physiological fluids such as urine, plasma, and serum both for inorganic and organic anions and cations. This application note outlines the analysis of physiological fluids using ion chromatography. Part one shows the anion method, the IonPac AS11 is coupled with the Anion Self-Regenerating Suppressor (ASRS) to dramatically reduce eluent conductivity, thus allowing the use of a rapidly increasing hydroxide gradient for the separation of an extensive group of monovalent through trivalent.

This Technical Note presents the advantages of the 4 μm particle-size IonPac AS11-HC-4μm column combined with the Dionex ICS-5000+ HPIC system for optimal separation of organic acids and inorganic anions in beer samples using electrolytically generated hydroxide eluent with and without an organic modifier. An ICS-6000 can be used for this application.