CAN108: Ion Chromatography Coupled with Mass Spectrometry for Metabolomics

Instrument Type: ICMS

Here, the author describes an ion-exchange system that provides good separation of the polar metabolities with on-line desalting to allow MS detection. This technique will allow studies of key metabolites that do not separate well on traditional reversed-phase columns. Some ionic metabolites may be positional isomers adjacent to each other in a synthetic pathway and as such, are isobaric. In such cases, MS is not sufficient to differentiate the two compounds. There is clearly a need for adequate separation techniques for isomeric metabolites.

AN162: Determination of Nucleotides by Ion Chromatography with UV Absorbance Detection.

Instrument Type: IC

The purpose of this application note is to provide a simple and direct assay of nucleotides and deoxynucleotides in amplification cocktails used for PCR, rolling circle amplifications, DNA and RNA polymerase reactions, and reverse-transcriptase reactions. Nucleotides are separated by anion exchange on the DNAPac PA100 column using gradient elution and detected by absorbance at 260 nm. Resolution of these components provides a means to assay their quality and help identify causes of amplification failure.