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AN162: Determination of Nucleotides by Ion Chromatography with UV Absorbance Detection.
Description
The purpose of this application note is to provide a simple and direct assay of nucleotides and deoxynucleotides in amplification cocktails used for PCR, rolling circle amplifications, DNA and RNA polymerase reactions, and reverse-transcriptase reactions. Nucleotides are separated by anion exchange on the DNAPac PA100 column using gradient elution and detected by absorbance at 260 nm. Resolution of these components provides a means to assay their quality and help identify causes of amplification failure.| Market: | BioPharma |
| Keywords: | Nucleotides, Visible Absorbance Detection, DNA, dATP, 2’-deoxyadenosine 5’–triphosphate, PCR reaction, amplification cocktails, DNAPac PA100, dGTP, dCTP, dTTP |
| Author: | David H. Thomas and Jeff Rohrer |
| Affiliation: | Thermo Fisher Scientific |
For Research Use Only. Not for use in diagnostic procedures.
