AB107: Determination of Hexavalent Chromium in Dyes

Instrument Type: IC

Hexavalent chromium [Cr(VI)] is the most toxic form of the metal chromium. Leather products colored with dyes containing trivalent chromium, and sweat can transfer the chromium from the leather to the skin. It is possible that some of the Cr(III) oxidizes to Cr(VI). Leather gloves were found to contain Cr(VI). Here, we analyze two dyes for Cr(VI) using a sensitive ion chromatography (IC) method with postcolumn reaction to produce a colored complex detected by visible absorbance. Also refer to AU 179 for an updated method for Cr(VI) analysis.

AN162: Determination of Nucleotides by Ion Chromatography with UV Absorbance Detection.

Instrument Type: IC

The purpose of this application note is to provide a simple and direct assay of nucleotides and deoxynucleotides in amplification cocktails used for PCR, rolling circle amplifications, DNA and RNA polymerase reactions, and reverse-transcriptase reactions. Nucleotides are separated by anion exchange on the DNAPac PA100 column using gradient elution and detected by absorbance at 260 nm. Resolution of these components provides a means to assay their quality and help identify causes of amplification failure.