AN163: Determination of Protein Concentrations Using AAA-Direct.
DescriptionIn this application note, we describe a method to determine total protein using AAA-Direct. We used this method to participate in an Association of Biomolecular Resource Facilities (ABRF) Amino Acid Analysis Research Group collaborative study that compared total protein determinations using AAA to dye-binding assays. We found that concentrations of all five protein solutions determined by AAA-Direct were equivalent to the determinations made by participants that used either pre- or postcolumn derivatization methods.
|Keywords:||AAA-Direct, Alanine, AminoPac PA10, Arginine, Electrochemical detection, Glycine, Histidine, IPAD, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Protein, Serine, Threonine, Tyrosine, Valine, total protein, Proline, Glutamate, Aspartate|
|Author:||Valoran Hanko and Jeff Rohrer|
|Affiliation:||Thermo Fisher Scientific|
For Research Use Only. Not for use in diagnostic procedures.