AN163: Determination of Protein Concentrations Using AAA-Direct.

Instrument Type: HPLC (Biocompatible)

In this application note, we describe a method to determine total protein using AAA-Direct. We used this method to participate in an Association of Biomolecular Resource Facilities (ABRF) Amino Acid Analysis Research Group collaborative study that compared total protein determinations using AAA to dye-binding assays. We found that concentrations of all five protein solutions determined by AAA-Direct were equivalent to the determinations made by participants that used either pre- or postcolumn derivatization methods.

High sensitivity analysis of amino acids by automated OPA derivatization on a Thermo Scientific Acclaim PolarAdvantage (PA) HPLC column

Instrument Type: HPLC

The Thermo Scientific Dionex UltiMate 3000 system is applied for the analysis of amino acids. The amino acids are derivatized in the autosampler with OPA before separation on a Thermo Scientific Acclaim PolarAdvantage (PA) 5 µm HPLC column. The derivative provides low detection limits using either UV or fluorescence detection.