AN163: Determination of Protein Concentrations Using AAA-Direct.

Instrument Type: HPLC (Biocompatible)

In this application note, we describe a method to determine total protein using AAA-Direct. We used this method to participate in an Association of Biomolecular Resource Facilities (ABRF) Amino Acid Analysis Research Group collaborative study that compared total protein determinations using AAA to dye-binding assays. We found that concentrations of all five protein solutions determined by AAA-Direct were equivalent to the determinations made by participants that used either pre- or postcolumn derivatization methods.

High sensitivity analysis of amino acids by automated OPA derivatization on a Thermo Scientific Acclaim PolarAdvantage (PA) HPLC column

Instrument Type: HPLC

The Thermo Scientific Dionex UltiMate 3000 system is applied for the analysis of amino acids. The amino acids are derivatized in the autosampler with OPA before separation on a Thermo Scientific Acclaim PolarAdvantage (PA) 5 µm HPLC column. The derivative provides low detection limits using either UV or fluorescence detection.

Improved separation of selected amino acids on a Thermo Scientific Acclaim Mixed-Mode WAX-1 column in HILIC mode

Instrument Type: HPLC

The Thermo Scientific Acclaim Mixed-Mode WAX-1 column is used for the separation of six selected amino acids, with UV detection at 210nm. The analysis is performed in HILIC mode, and the amino acids elute in order of most hydrophobic to least hydrophobic.