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Fast analysis of glutamate in beer by automated precolumn OPA derivatization

Instrument Type: HPLC

The Thermo Scientific HPLC system is applied for the analysis of glutamate in beer. The sample preparation is automated, and the separation is performed using a Thermo Scientific Acclaim PolarAdvantage column, with UV detection at 330 nm. Chromatogram A is beer and chromatogram B is beer spiked with 250 µg/mL glutamic acid.

AN163: Determination of Protein Concentrations Using AAA-Direct.

Instrument Type: HPLC (Biocompatible)

In this application note, we describe a method to determine total protein using AAA-Direct. We used this method to participate in an Association of Biomolecular Resource Facilities (ABRF) Amino Acid Analysis Research Group collaborative study that compared total protein determinations using AAA to dye-binding assays. We found that concentrations of all five protein solutions determined by AAA-Direct were equivalent to the determinations made by participants that used either pre- or postcolumn derivatization methods.