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Displaying 1-3 of 3 results for Tag: Lysine
AN163: Determination of Protein Concentrations Using AAA-Direct.Instrument Type: HPLC (Biocompatible)
In this application note, we describe a method to determine total protein using AAA-Direct. We used this method to participate in an Association of Biomolecular Resource Facilities (ABRF) Amino Acid Analysis Research Group collaborative study that compared total protein determinations using AAA to dye-binding assays. We found that concentrations of all five protein solutions determined by AAA-Direct were equivalent to the determinations made by participants that used either pre- or postcolumn derivatization methods.
High sensitivity analysis of amino acids by automated OPA derivatization on a Thermo Scientific Acclaim PolarAdvantage (PA) HPLC columnInstrument Type: HPLC
The Thermo Scientific Dionex UltiMate 3000 system is applied for the analysis of amino acids. The amino acids are derivatized in the autosampler with OPA before separation on a Thermo Scientific Acclaim PolarAdvantage (PA) 5 µm HPLC column. The derivative provides low detection limits using either UV or fluorescence detection.
Improved monitoring of monoclonal antibody heterogeneity by cation-exchange chromatography using a Thermo Scientific ProPac WCX-10 columnInstrument Type: HPLC (Biocompatible)
The Thermo Scientific UltiMate 3000 titanium system is applied for analyzing a humanized IgG1 MAb for C-terminal lysine variants. The separation was performed on a Thermo Scientific ProPac WCX-10 column, with UV detection at 280 nm.