Quick analysis of arginine and methylated arginines by LC-MS

Instrument Type: LCMS

The Thermo Scientific HPLC system is applied for the analysis of arginine and methylated arginines. The separation is performed on a Thermo Scientific Hypercarb column, with MS detection.

AN163: Determination of Protein Concentrations Using AAA-Direct.

Instrument Type: HPLC (Biocompatible)

In this application note, we describe a method to determine total protein using AAA-Direct. We used this method to participate in an Association of Biomolecular Resource Facilities (ABRF) Amino Acid Analysis Research Group collaborative study that compared total protein determinations using AAA to dye-binding assays. We found that concentrations of all five protein solutions determined by AAA-Direct were equivalent to the determinations made by participants that used either pre- or postcolumn derivatization methods.

Simple HILIC separation of arginine and glutamic acid without derivatization using HPLC-UV

Instrument Type: HPLC

The Thermo Scientific Dionex Ultimate 3000 system is applied for the analysis of two amino acids. The separation or arginine and glutamic acid was performed in less than 10 minutes on a Thermo Scientific Acclaim Mixed-Mode HILIC-1 HPLC column without the need for derivatization.

High sensitivity analysis of amino acids by automated OPA derivatization on a Thermo Scientific Acclaim PolarAdvantage (PA) HPLC column

Instrument Type: HPLC

The Thermo Scientific Dionex UltiMate 3000 system is applied for the analysis of amino acids. The amino acids are derivatized in the autosampler with OPA before separation on a Thermo Scientific Acclaim PolarAdvantage (PA) 5 µm HPLC column. The derivative provides low detection limits using either UV or fluorescence detection.