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TN40: Glycoprotein Monosaccharide Analysis Using HPAE-PAD with Eluent Generation Be the first to rate this application

tn40 glycoprotein monosaccharide analysis using hpaepad with eluent generation

Description

Characterization of glycoproteins routinely involves carbohydrate analysis. Studies show that HPAE-PAD is a simple and effective way to determine glycoprotein monosaccharide composition without derivatization. In a typical method, monosaccharides are separated on a Thermo Scientific Dionex CarboPac PA10 or PA20 anion-exchange column, preceded by a Thermo Scientific Dionex AminoTrap guard column, using a hydroxide eluent prepared manually or generated by an eluent generator. This TN reports such a method.
Market: BioPharma
Keywords: AminoTrap, CarboPac PA20, electrochemical detection, fucose, galactosamine, galactose, glucosamine, glucose, glycoprotein, HPAE-PAD, mannose, monosaccharide, PAD
Matrix: Fetuin, Glycoprotein acid digest
Author: Lipika Basumallick and Jeffrey Rohrer
Affiliation: Thermo Fisher Scientific
Uploaded on 10/5/2015.

For Research Use Only. Not for use in diagnostic procedures.