This work describes an HPAE-PAD method for monosaccharide composition analysis using manually prepared eluent. Monosaccharide analysis using electrolytically generated eluent has been described before. Here, three commercially available proteins, IgG, fetuin, and alpha-1-acid glycoprotein, were individually subjected to two hydrolysis conditions using 1)HCl, for the amino sugars galactosamine and glucosamine, and 2)TFA, for the neutral sugars mannose, glucose, and galactose. Results for method linearity, robustness, and accuracy are presented here. An ICS-6000 can be used for this application.

HPAE-PAD is widely used for determination of carbohydrates by measuring the electrical current generated by their oxidation at the surface of a gold electrode. Each amperometric detection cell includes an amperometric working electrode exposed to a sample flow-through channel, typically enclosed by a plastic gasket held in place by compression. Here, the sensitivity and linearity of six carbohydrates have been evaluated using 2, 15, and 62 mil gaskets under typical chromatographic conditions used for Thermo Scientific™ Dionex™ CarboPac™ SA10 column with and without postcolum addition.

HPAE-PAD is widely used for determination of carbohydrates by measuring the electrical current generated by their oxidation at the surface of a gold electrode. Each amperometric detection cell includes an amperometric working electrode exposed to a sample flow-through channel, typically enclosed by a plastic gasket held in place by compression. Here, the sensitivity and linearity of six carbohydrates have been evaluated using 2, 15, and 62 mil gaskets under typical chromatographic condition used for Thermo Scientific™ Dionex™ CarboPac™ PA20 column.

The method uses the Thermo Scientific™ Dionex™ CarboPac™ PA20 column with electrolytically generated hydroxide eluent and a 15 mil gasket for the gold working electrode to separate and detect glucose, fructose and sucrose in kombucha.

本文使用毛细管离子色谱-安培检测研究了枸杞多糖 的单糖组成，对其中几种低含量单糖的浓度也可进行准 确测定。对水解使用酸种类和浓度、水解温度和时间等 前处理条件进行了考察优化，并结合离子色谱糖分析柱 对单糖的良好分离能力和脉冲安培检测的高灵敏度，可 以对枸杞多糖中单糖组分进行更全面的剖析。本文采用 的测定方法也可以广泛用于其他物质中多糖的检测，为 多糖结构研究提供更全面有效的信息。