This is the first document showing the use of eluent generation for HPAE-PAD monosaccharide analysis of glycoproteins. Those interested in an updated version should see TN40. This record is provided for those with an EG40 and a Dionex CarboPac PA10 column that would like to do this application. This document also has useful sample preparation information for HPAE-PAD glycorprotein monosaccharide analysis.

Characterization of glycoproteins routinely involves carbohydrate analysis. Studies show that HPAE-PAD is a simple and effective way to determine glycoprotein monosaccharide composition without derivatization. In a typical method, monosaccharides are separated on a Thermo Scientific Dionex CarboPac PA10 or PA20 anion-exchange column, preceded by a Thermo Scientific Dionex AminoTrap guard column, using a hydroxide eluent prepared manually or generated by an eluent generator. This TN reports such a method.

Wood, grass, and other lignocellulosic biomass are emerging as the preferred sources for biofuel and biochemical production. Compositional analysis of plant derived material is crucial for maximizing the yield of biofuel production. A single HPAE-PAD method, which uses the Dionex CarboPac PA20 column, can be used for the simultaneous determination of mono- and disaccharides and uronic acids.

A fast method using the Dionex CarboPac SA10 column was tested for determining the common coffee carbohydrates. This column achieves fast, high resolution separation of mono- and disaccharides commonly found in food samples. The Dionex CarboPac SA10 column is composed of a wide-pore macroporous substrate coated with a strong anion-exchange latex of nano-beads. The combination of the high capacity provided by the substrate and the new internal chemistry of the nano-bead functionality delivers high resolution and short analysis time for the common sugars of interest in food and beverages.

This study shows the AOAC official method 995.13 on a Thermo Scientific Dionex ICS-3000 system. Carbohydrates in extracts from instant coffee and green coffee beans were separated on a Dionex CarboPac PA1 column, and measured by electrochemical detection with disposable Au on polytetrafluoroethylene (PTFE) working electrodes. A few proposed modifications of the official method achieved separation of two pairs of sugars, which are otherwise difficult to resolve.