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TN40: Glycoprotein Monosaccharide Analysis Using HPAE-PAD with Eluent Generation
Description
Characterization of glycoproteins routinely involves carbohydrate analysis. Studies show that HPAE-PAD is a simple and effective way to determine glycoprotein monosaccharide composition without derivatization. In a typical method, monosaccharides are separated on a Thermo Scientific Dionex CarboPac PA10 or PA20 anion-exchange column, preceded by a Thermo Scientific Dionex AminoTrap guard column, using a hydroxide eluent prepared manually or generated by an eluent generator. This TN reports such a method.| Market: | BioPharma |
| Keywords: | AminoTrap, CarboPac PA20, Electrochemical detection, fucose, Glucose, glycoprotein, Mannose, PAD, galactosamine, monosaccharide, galactose, glucosamine, HPAE-PAD, TN21, TN71, TN110, TN 71, TN40, TN 125, TN 20, TN 70671, TN20, TN70671, TN125, TN 21, TN 110, TN 40 |
| Matrix: | Fetuin, Glycoprotein acid digest |
| Author: | Lipika Basumallick and Jeffrey Rohrer |
| Affiliation: | Thermo Fisher Scientific, Sunnyvale, CA USA |
For Research Use Only. Not for use in diagnostic procedures.
