Determination of Glucosamine in Dietary Supplements Using a Compact Ion Chromatography System

Instrument Type: IC

This record shows the rapid, rugged HPAE-PAD method for determining glucosamine in dietary supplement tablets, gelatin capsules, and fortified liquids that was published in Application Note 197. In this record the method is executed on an Integrion ion chromatography system.

TN53: Determination of Glycoprotein Monosaccharide Composition by HPAE-PAD Using On-Line Electrolytically Generated Eluents

Instrument Type: IC

This is the first document showing the use of eluent generation for HPAE-PAD monosaccharide analysis of glycoproteins. Those interested in an updated version should see TN40. This record is provided for those with an EG40 and a Dionex CarboPac PA10 column that would like to do this application. This document also has useful sample preparation information for HPAE-PAD glycorprotein monosaccharide analysis.

TN40: Glycoprotein Monosaccharide Analysis Using HPAE-PAD with Eluent Generation

Instrument Type: IC

Characterization of glycoproteins routinely involves carbohydrate analysis. Studies show that HPAE-PAD is a simple and effective way to determine glycoprotein monosaccharide composition without derivatization. In a typical method, monosaccharides are separated on a Thermo Scientific Dionex CarboPac PA10 or PA20 anion-exchange column, preceded by a Thermo Scientific Dionex AminoTrap guard column, using a hydroxide eluent prepared manually or generated by an eluent generator. This TN reports such a method.

AN197: Determination of Glucosamine in Dietary Supplements Using HPAE-PAD

Instrument Type: IC

Glucosamine (GlcN), a naturally occurring amino sugar, is a major structural component in the biosynthesis of glycosaminoglycans. Use of GlcN as a dietary supplement in the management of osteoarthritis has attracted considerable attention. Pulsed amperometric detection (PAD) is sensitive and selective and therefore ideally suited for determination of GlcN. High-performance anion-exchange with pulsed amperometric detection (HPAE-PAD) chromatography is a sensitive, direct-detection technique capable of separating mono- and disaccharides rapidly and efficiently.