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Assay for water-soluble vitamins in vitamin tablets using a polar-embedded HPLC column
Description
The Thermo Scientific HPLC system is applied for the determination of water-soluble vitamins in vitamin tablets. The separation is performed on a Thermo Scientific Acclaim PolarAdvantage (PA) HPLC column, with UV detection at various wavelengths. Some vitamins are strongly hydrophilic, so the column needs to operate in 100% aqueous buffer to gain sufficient retention to resolve them from the matrix. The Acclaim PA can do this reliably whereas a hydrophobic C18 column would likely suffer dewetting.| Market: | Food and Beverage |
| Keywords: | Acclaim PolarAdvantage (PA), Ascorbic acid, Folic acid, HPLC, Niacinamide, Pantothenic acid, Pyridoxine, Riboflavin, Thiamine, UV detection, Vitamin B12, Water-soluble vitamins |
| Matrix: | Tablet (vitamin) |
For Research Use Only. Not for use in diagnostic procedures.
