• To demonstrate the capability of the Thermo Scientific™ Acclaim™ PolarAdvantage II (PA2) and Thermo Scientific™ Vanquish™ Flex Binary UHPLC system combination for the rapid separation of water soluble vitamins in sports beverages with excellent linearity, reproducibility, resolution, and recoveries. • To show the capability of the Vanquish Flex Binary UHPLC system to provide superior retention time precision through its novel pump features.

The Thermo Scientific Dionex UltiMate 3000 RS system is applied for the analysis of electrolytes in sports beverages. The separation is performed on a Thermo Scientific Acclaim Trinity P2 column, which is designed to resolve a broad range of anions and cations, mono- or multi-valent, in a single analysis using a simple gradient method. The Thermo Scientific OnGuard II P cartridge was used to remove artificial colors from the samples.

The Thermo Scientific HPLC system is applied for the analysis of organic acids in orange juice. The separation is performed on a Thermo Scientific Acclaim Organic Acid (OA) column, with UV detection at 210 nm. The samples were prepared using Thermo Scientific Dionex OnGuard II P SPE cartridges.

The Thermo Scientific Dionex UltiMate 3000 RSLC is applied for the analysis of water soluble vitamins from soft drink. The separation was performed in less than 5 minutes on a Thermo Scientific Acclaim RSLC PolarAdvantage II (PA2) 2.2 µm column with UV detection.

The Thermo Scientific Dionex UltiMate 3000 RS system is applied for the analysis of seven water-soluble vitamins. The separation was performed in less than 12 minutes on a Thermo Scientific Acclaim C30 5 µm column, with UV detection at 210 nm.