• To demonstrate the capability of the Thermo Scientific™ Acclaim™ PolarAdvantage II (PA2) and Thermo Scientific™ Vanquish™ Flex Binary UHPLC system combination for the rapid separation of water soluble vitamins in sports beverages with excellent linearity, reproducibility, resolution, and recoveries. • To show the capability of the Vanquish Flex Binary UHPLC system to provide superior retention time precision through its novel pump features.

The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the separation of multivitamins (Folic Acid,Vitamins C, B12, and E from a Multivitamin Capsule. The separation was performed on a Thermo Scientific Acclaim Polar Advantage-II HPLC column with a UV detection at 270 nm. With a run time of 35 minutes, the method allows easy separation of Vitamin C, B12, Folic Acid and E from Multivitamin Capsule.

The Thermo Scientific Dionex UltiMate 3000 RSLC is applied for the analysis of water soluble vitamins from soft drink. The separation was performed in less than 5 minutes on a Thermo Scientific Acclaim RSLC PolarAdvantage II (PA2) 2.2 µm column with UV detection.

The Thermo Scientific Dionex UltiMate 3000 RS system is applied for the analysis of seven water-soluble vitamins. The separation was performed in less than 12 minutes on a Thermo Scientific Acclaim C30 5 µm column, with UV detection at 210 nm.

The Thermo Scientific Ultimate 3000 RSLC system is applied for the determination of water-soluble vitamins. Seven vitamins were separated using the Thermo Scientific Acclaim Trinity P1 column with a run time of less than 10 minutes