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Displaying 1-2 of 2 results for Tag: Vitamin B12

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Separation of Vitamin C, Vitamin B12, Folic Acid and Vitamin-E from Multivitamin Capsules.

Instrument Type: HPLC

The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the separation of multivitamins (Folic Acid,Vitamins C, B12, and E from a Multivitamin Capsule. The separation was performed on a Thermo Scientific Acclaim Polar Advantage-II HPLC column with a UV detection at 270 nm. With a run time of 35 minutes, the method allows easy separation of Vitamin C, B12, Folic Acid and E from Multivitamin Capsule.

Assay for water-soluble vitamins in vitamin tablets using a polar-embedded HPLC column

Instrument Type: HPLC

The Thermo Scientific HPLC system is applied for the determination of water-soluble vitamins in vitamin tablets. The separation is performed on a Thermo Scientific Acclaim PolarAdvantage (PA) HPLC column, with UV detection at various wavelengths. Some vitamins are strongly hydrophilic, so the column needs to operate in 100% aqueous buffer to gain sufficient retention to resolve them from the matrix. The Acclaim PA can do this reliably whereas a hydrophobic C18 column would likely suffer dewetting.