This application note discusses improvements made to the previous method. One change involves mobile phase optimization, in which alternative organic modifiers are studied. Another change is the addition of a wash step, which prevents carryover of the sample matrix between runs. Adjustments made to the SPE provide a much higher sample recovery. The internal standard is also changed to ProG in order to prevent elution with matrix interferents.

The Thermo Scientific HPLC system is applied for the analysis of phenol standards by EPA method 604. The separation is performed on a Thermo Scientific Acclaim PolarAdvantage (PA) column, with UV detection at 285 nm.

The Thermo Scientific HPLC system is applied for the analysis of nitrosamines in EPA 8270. The separation is performed on a Thermo Scientific Acclaim PolarAdvantage (PA) column, with UV detection at 230 nm.

The Thermo Scientific HPLC system is applied for the analysis of propafenone and related substances. The separation is performed using a Thermo Scientific Acclaim PolarAdvantage column, with UV detection at 249 nm.

The Thermo Scientific HPLC system is applied for the impurity profile of 2-(N,N-dimethylamino)ethyl acrylate. The separation is performed using a Thermo Scientific Acclaim PolarAdvantage column, with UV detection at 210 nm. The three chromatograms represent three different batches of DMAEA.