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Influence of Column Temperature on Reversed-Phase Chromatography of an Intact Antibody Be the first to rate this application


The Thermo Scientific Vanquish Flex UHPLC System was used in combination with the MAbPac RP column for the reversed-phase analysis of intact bevacizumab. Column thermostatting strongly influences peak shape and recovery. For runs with column temperature set between 60 and 90 °C, peak width at half height is below 3 seconds for a 30 minutes long gradient. The largest peak area is obtained at 70 °C, and decreases steeply at higher and lower column temperatures. Carry-over decreases at higher temperature. At 70 °C the carry-over is estimated at 0.37%.
Market: BioPharma
Keywords: Biotherapeutics, Monoclonal antibody, Biopharmaceutical, MAbPac RP, Biocompatible UHPLC, Vanquish Flex UHPLC System, Bevacizumab, Avastin
Matrix: Water
Author: Michael Menz
Affiliation: Thermo Fisher Scientific, Germering, Germany
Uploaded on 2/1/2016.

For Research Use Only. Not for use in diagnostic procedures.