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Displaying 1-3 of 3 results for Tag: Vanquish Flex UHPLC System

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A rapid ibuprofen USP assay method

Instrument Type: UHPLC

To demonstrate practical approaches that can be used to significantly improve throughput of the ibuprofen USP assay monograph keeping to the spirit o USP-NF Chapter <621> guidelines while maintaining USP quality acceptance criteria. To then take this optimized assay monograph and reduce analysis time even further.

Rapid and sensitive UHPLC screening of food dyes in carbonated beverages using UV/Vis wavelength switching

Instrument Type: UHPLC

The analysis of food dyes in carbonated beverages is important as many of these dyes are either controlled substances or are being phased out in certain countries due to their reported adverse health effects. Being able to identify and quantify food dyes in beverages quickly and with high sensitivity is therefore important. Reversed-phase chromatography is an excellent technique for the analysis of dyes. Many dyes are readily soluble in reversed-phase eluents and have strong visible and UV absorbance properties. This method demonstrates the separation of ten contentious dyes.

Influence of Column Temperature on Reversed-Phase Chromatography of an Intact Antibody

Instrument Type: HPLC (Biocompatible)

The Thermo Scientific Vanquish Flex UHPLC System was used in combination with the MAbPac RP column for the reversed-phase analysis of intact bevacizumab. Column thermostatting strongly influences peak shape and recovery. For runs with column temperature set between 60 and 90 °C, peak width at half height is below 3 seconds for a 30 minutes long gradient. The largest peak area is obtained at 70 °C, and decreases steeply at higher and lower column temperatures. Carry-over decreases at higher temperature. At 70 °C the carry-over is estimated at 0.37%.