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Description
High-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) overcomes the limitations other methods and delivers fast carbohydrate analysis. However, due to the high concentration of the biofuel samples, a several-fold sample dilution must be performed before analysis, introducing a source of error. In this study, 2 hardware modifications were applied to handle high-concentration samples: A reduced injection volume from 10 to 0.4 μL and an increase in spacer gasket thickness in the electrochemical flow cell to 62 mil to reduce detection sensitivity.| Market: | Chemical |
| Keywords: | arabinose, carbohydrate, disaccharide, Electrochemical detection, Glucose, xylose, Mannose, Sucrose, PAD, Cellobiose, fructose, 62 mil gasket, biomass, CarboPac SA10, monosaccharide, galactose, HPAE-PAD, TN21, TN110, TN 20, TN 70671, TN20, TN70671 |
| Matrix: | Hydrolyzed corn stover |
| Author: | Lipika Basumallick and Jeff Rohrer |
| Affiliation: | Thermo Fisher Scientific, Sunnyvale, CA USA |
For Research Use Only. Not for use in diagnostic procedures.
