This work updates the column used in AN1161 with a shorter 4 × 150 mm format column. The combination of the smaller resin particle size of the Dionex CarboPac SA10-4μm column and the shorter column used here results in separation of eight common sugars in less than six minutes. This is significant time savings as compared to the eight-minute runtime achieved in AN1089. The shorter run time allows for faster sample turnaround times and reduced eluent consumption, thereby improving the overall process economics. Results for method linearity, accuracy, and robustness are presented here.

The Accelerated Solvent Extraction (ASE) provides a fast and efficient way to extract various biomass samples for sugar analysis to determine sample viability for alcohol production. This application note describes methods for the extraction of sugars from biomass samples using acidified solvents (in-cell hydrolysis) and extracting pre-hydrolyzed samples.

The current work updates the system used for the carbohydrate analysis in AN1089. The new system combines flexibility and ease-of-use with high sensitivity and selectivity, bringing a higher level of convenience and cost effectiveness to simple sugar analysis. The method proposed here separates eight common carbohydrate sugars in less than eight minutes. The shorter run time results in better process economics. Using this method, carbohydrates present in 10 individual biofuel samples were quantified.

High-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) overcomes the limitations other methods and delivers fast carbohydrate analysis. However, due to the high concentration of the biofuel samples, a several-fold sample dilution must be performed before analysis, introducing a source of error. In this study, 2 hardware modifications were applied to handle high-concentration samples: A reduced injection volume from 10 to 0.4 μL and an increase in spacer gasket thickness in the electrochemical flow cell to 62 mil to reduce detection sensitivity.

This work describes a high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD)-based method for the determination of HMF in samples ranging from food to treated biomass. A Dionex ICS-3000 system with a CarboPac PA1 column, electrolytically generated hydroxide eluent, and electrochemical detection with disposable Au-on-polytetrafluoroethylene working electrodes are used. The Dionex CarboPac PA1 is a high-capacity, rugged column suitable for determining mono- and disaccharides, and has high resolution for HMF in a wide variety of matrices.