TN186: The Effect of Working Electrode Gasket Thickness on the Sensitivity and Linearity of Carbohydrate Response by PAD with the Dionex™ CarboPac™ SA10 Column

Instrument Type: IC

HPAE-PAD is widely used for determination of carbohydrates by measuring the electrical current generated by their oxidation at the surface of a gold electrode. Each amperometric detection cell includes an amperometric working electrode exposed to a sample flow-through channel, typically enclosed by a plastic gasket held in place by compression. Here, the sensitivity and linearity of six carbohydrates have been evaluated using 2, 15, and 62 mil gaskets under typical chromatographic conditions used for Thermo Scientific™ Dionex™ CarboPac™ SA10 column with and without postcolum addition.

TN186: The Effect of Working Electrode Gasket Thickness on the Sensitivity and Linearity of Carbohydrate Response by PAD with the Dionex™ CarboPac™ PA20 Column

Instrument Type: IC

HPAE-PAD is widely used for determination of carbohydrates by measuring the electrical current generated by their oxidation at the surface of a gold electrode. Each amperometric detection cell includes an amperometric working electrode exposed to a sample flow-through channel, typically enclosed by a plastic gasket held in place by compression. Here, the sensitivity and linearity of six carbohydrates have been evaluated using 2, 15, and 62 mil gaskets under typical chromatographic condition used for Thermo Scientific™ Dionex™ CarboPac™ PA20 column.

AU192: Carbohydrate Determination of Biofuel Samples

Instrument Type: IC

High-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) overcomes the limitations other methods and delivers fast carbohydrate analysis. However, due to the high concentration of the biofuel samples, a several-fold sample dilution must be performed before analysis, introducing a source of error. In this study, 2 hardware modifications were applied to handle high-concentration samples: A reduced injection volume from 10 to 0.4 μL and an increase in spacer gasket thickness in the electrochemical flow cell to 62 mil to reduce detection sensitivity.

AN282: Rapid and Sensitive Determination of Biofuel Sugars by Ion Chromatography

Instrument Type: IC

This work shows a rapid (<8 min) and robust method with minimal sample pretreatment using high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) to determine the sugars of interest in the highly concentrated samples encountered in biofuel processing.