The method uses the Thermo Scientific™ Dionex™ CarboPac™ PA20 column with electrolytically generated hydroxide eluent and a 15 mil gasket for the gold working electrode to separate and detect glucose, fructose and sucrose in kombucha.

This record demonstrates the determinations of glucose, fructose, and sucrose in a 100-fold diluted flavored rum alcohol sample by HPAE-PAD. Typically samples with g/L concentrations require > 10,000-fold dilutions to remain in the linear range of the very sensitivie HPAE-PAD technique. However, this method, demonstrated on the Integrion RFIC system, uses a 0.4 µL internal injection loop with the High Concentration Carbohydrate Analysis Kit to extend the linearity from low mg/L to g/L concentrations. A wood hydrolysate application using this technique is attached for reference.

This record demonstrates the determinations of glucose and fructose in a 100-fold diluted rice wine sample by HPAE-PAD. Typically samples with g/L concentrations require > 10,000-fold dilutions to remain in the linear range of the very sensitive HPAE-PAD technique. However, this method, demonstrated on the Integrion RFIC system, uses a 0.4 µL internal injection loop with the High Concentration Carbohydrate Analysis Kit to extend the linearity from low mg/L to g/L concentrations. A wood hydrolysate application using this technique is attached for reference.

This record demonstrates the determinations of glucose and fructose in a 100-fold diluted basalmic vinegar sample by HPAE-PAD. Typically samples with g/L concentrations require >10,000-fold dilutions to remain in the linear range of the very sensitive HPAE-PAD technique. However, this method demonstrated on the Integrion RFIC system, uses a 0.4 µL internal injection loop with the High Concentration Carbohydrate Analysis Kit to extend the linearity from low mg/L to g/L concentrations. A wood hydrolysate application using this technique is attached for reference.

This record demonstrates the determinations of glucose, fructose, and sucrose in a 100-fold diluted sparkling wine sample by HPAE-PAD. Typically samples with g/L concentrations require > 10,000-fold dilutions to remain in the linear range of the very sensitive HPAE-PAD technique. However, this method demonstrated on the Integrion RFIC system, uses a 0.4 µL internal injection loop with the High Concentration Carbohydrate Analysis Kit to extend the linearity from low mg/L to g/L concentrations. A wood hydrolysate application using this technique is attached for reference.