AU192: Carbohydrate Determination of Biofuel Samples

Instrument Type: IC

High-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) overcomes the limitations other methods and delivers fast carbohydrate analysis. However, due to the high concentration of the biofuel samples, a several-fold sample dilution must be performed before analysis, introducing a source of error. In this study, 2 hardware modifications were applied to handle high-concentration samples: A reduced injection volume from 10 to 0.4 μL and an increase in spacer gasket thickness in the electrochemical flow cell to 62 mil to reduce detection sensitivity.

AN1091: Determination of Uronic Acids and Wood Sugars in Wood-Based Hydrolysates: Comprehensive Method

Instrument Type: IC

Wood, grass, and other lignocellulosic biomass are emerging as the preferred sources for biofuel and biochemical production. Compositional analysis of plant derived material is crucial for maximizing the yield of biofuel production. A single HPAE-PAD method, which uses the Dionex CarboPac PA20 column, can be used for the simultaneous determination of mono- and disaccharides and uronic acids.

AN225: Rapid Method for the Estimation of Total Free Monosaccharide Content of Corn Stover Hydrolysate Using HPAE-PAD

Instrument Type: IC

Corn stover, an acid-hydrolyzed leaf, husk, stalk, and cob remenants in the field after harvest, is a common feedstock for fermentation systems used in biofuel production. HPAE-PAD is a well-accepted method for carbohydrate determinations such as those found corn stover. The CarboPac® PA1 anion-exchange column rapidly elutes corn stover carbohydrates, resolving the monosacccharides from the unretained and undetected noncarbohydrate components of acid-hydrolyzed corn stover. Under these conditions, disaccharides and trisaccharides are also resolved, with a total elution time less than 10 min.