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Analysis of Protein Therapeutics and Excipients using 2D Chromatography with Combined UV and Charged Aerosol Detection
Description
Therapeutic proteins (antibodies and vaccines) vary considerably and unwanted aggregation is a major path to degradation. Stabilization of protein formulations can be enhanced through the addition of specific excipients such as amino acids, ions, surfactants and sugars. A 2D “heart cut” technique allows the separation of the protein component of a commercial therapeutic formulation and analyzed using a DAD detector and polar sugar and amino acid excipients to be separated on the second dimension using ion-pairing RP conditions and detected via the charged aerosol detector.| Market: | BioPharma |
| Matrix: | Water |
| Author: | Bruce Bailey |
| Affiliation: | Thermo Scientific |
For Research Use Only. Not for use in diagnostic procedures.
