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AN1050: Evaluating Protein Glycosylation in Limited-Quantity Samples by HPAE-PAD - Oligosaccharide Method
Description
By using a combination of acid hydrolysis and enzymatic digestion steps prior to HPAE-PAD analysis, information about a protein's glycosylation, as well as linkages within the glycans, is determined. These methods provide a cost-effective option for investigating glycans without the derivatization required by many other methods. This record details a method for oligosaccharide analysis.| Market: | BioPharma |
| Keywords: | glycoprotein, oligosaccharide, PAD, CarboPac PA200, HPAEC-PAD, PNGase F, PSA, sialylated, IgG, HPAE-PAD, TN71, TN 71, TN 20, TN 70671, TN20, TN70671, A0F, A1F, charged glycans, Neutral glycans, G0F, G1F, G2bF, Man3, Man6, G0, G0bF, G1bF, Man9 |
| Matrix: | Glycoprotein |
| Author: | Deanna Hurum, Lipika Basumallick, and Jeff Rohrer |
| Affiliation: | Thermo Fisher Scientific, Sunnyvale, CA USA |
For Research Use Only. Not for use in diagnostic procedures.
