AN1050: Evaluating Protein Glycosylation in Limited-Quantity Samples by HPAE-PAD - Oligosaccharide Method

Instrument Type: IC

By using a combination of acid hydrolysis and enzymatic digestion steps prior to HPAE-PAD analysis, information about a protein's glycosylation, as well as linkages within the glycans, is determined. These methods provide a cost-effective option for investigating glycans without the derivatization required by many other methods. This record details a method for oligosaccharide analysis.

AN1050: Evaluating Protein Glycosylation in Limited-Quantity Samples by HPAE-PAD, Monosaccharide Method

Instrument Type: IC

HPAE-PAD methods provide a cost-effective option for investigating glycans without the derivatization required by many other methods. Electrochemical detection determines the glycans directly without the potential loss of sialylation or linkage rearrangement that may occur during analysis with other methods. HPAE-PAD can be used for determining monosaccharides, oligosaccharides, sialic acids, and other carbohydrates. This record shows a monosaccharide method.