AN1149: Profiling Fructosyloligosaccharides (FOS)-containing samples by HPAE-PAD

Instrument Type: IC

In this work, the oligo- and polysaccharide distribution of three different inulin samples has been characterized by HPAE-PAD. In addition, HPAE-PAD profiling was demonstrated as a method to determine FOS content in prebiotic samples and variation in prebiotics on addition of probiotics. We used Inulin-FOS and chicory root inulin as prebiotic samples. For probiotics, we used Yakult drink, a well known probiotic dairy product. In this method, separation of individual oligosaccharides in FOS was achieved on a Dionex CarboPac PA200 column. An ICS-6000 can be used for this application.

AN1050: Evaluating Protein Glycosylation in Limited-Quantity Samples by HPAE-PAD - Oligosaccharide Method

Instrument Type: IC

By using a combination of acid hydrolysis and enzymatic digestion steps prior to HPAE-PAD analysis, information about a protein's glycosylation, as well as linkages within the glycans, is determined. These methods provide a cost-effective option for investigating glycans without the derivatization required by many other methods. This record details a method for oligosaccharide analysis.